Difference Between C3 Plants and C4 Plants: The following are the points of difference between C3 plants and C4 plants– C3 plants C4 plants They are mainly temperate plants. They are mainly tropical plants. They operate only the Calvin cycle. They operate the Calvin cycle as well as the Hatch and Slack pathway. The optimum temperature for photosynthesis is 20°-25°C. The optimum temperature for photosynthesis is 30°-45°C. The transpiration ratio is 500-1000. The transpiration ratio is 250-350. CO₂ fixation takes place in mesophyll cells only. CO₂ fixation takes place in mesophyll and bundle sheath. Chloroplasts are granal (one type). Chloroplasts … Continue reading Difference Between C3 Plants And C4 Plants